UJI AKTIVITAS PENGHAMBATAN ENZIM α-GLUKOSIDASE EKSTRAK ETANOL BIJI BUAH ALPUKAT SANGRAI (Persea americana Mill.) SECARA IN VITRO

St. Maryam Maryam, Asriani Suhaenah, Nurul Fadhillah Amrullah

Abstract


It is known that the seeds of avocado (Persea americana Mill.) are one part of the avocado widely used to treat various diseases, including diabetes. Based on the previous research, avocado seeds contain flavonoids with the potency as inhibitors of the enzyme α-glucosidase. The research aimed to determine the potency of ethanol extracted by meceration using 96% ethanol. The inhibitory activity was assayed using ELISA (Enzyme-Linked Immunosorbant Assay) reader measured at the wavelength of 405 with an acarbose comparator. The results showed that the ethanol extract of avocado seeds had an inhibitory activity included in the active category based on IC50 value of 34.300 µg/mL. Meanwhile, the acarbose had inhibition activity included in the very active category with an IC50 value of 0.253 µg/mL.


Keywords


Avocado seeds (Persea americana Mill.); Enzyme α-Glucosidase;ELISA (Enzyme-Linked Immunosorbant Assay) reader.

References


Corwin and Elizabeth J. Buku saku Patofisiologi edk 3. Jakarta :EGC, 2009. pp 624.

Si MM, Lou JS, Zhou CX Shen JN, Wu HH, Yang B, He QJ and Wu HS. Insulin releasing and alpha-glucosidase inhibitory activity of ethyl acetate fraction of Acorus calamus in vitro and in vivo. Journal of Ethnopharmacology. 2010;128:(154-159).

Marlinda M, Sangi M and Wuntu AD. Analisis Senyawa Metabolit Sekunder dan Uji Toksisitas Ekstrak Etanol Biji Buah Alpukat (Persea americana Mill.). Journal MIPA UNSRAT.2012;1(1):24-28.

Yuefei W, Shuangru H, Shuhong S, Lisheng Q and Ping X. Studies on bioactivities of tea (Camellia sinensis L.) fruit peel extracts: Antioxidant activity and inhibitory potential against alpha-glucosidase and alpha- amylase in vitro. Industrial Crops and Products. 2012;37(1):520-526.

Tadera K, Minami Y, Takamatsu K and Matsuoka. Inhibition of alpha-Glucosidae and alpha-amylase by Flavonoids. Journal of Nutritional Science and Vitaminology. 2006;52(2):149-153.

Shinde J, Taldone T, Barletta M, Kunaparaju N, Bo H and Kumar S. Alpha-Glucosidase inhibitory activity of Syzygium cumini (Linn.) Skeels seed kernel in vitro and in Goto–Kakizaki (GK) rats’. Carbohydrate Research. 2008;343(7):1278-1279.

Nurhijrah. Uji aktivitas penghambatan Enzim alfa-glukosidase ekstrak metanol biji alpukat (Persea americana Mill.). menggunakan ELISA reader (Skripsi). Makassar: Fakultas Farmasi, Universitas Muslim Indonesia, 2016.

Layla Z and Poerwadikarta MB. Teknik Uji Aglutinasi cepat dan Enzyme Linked Immunosorbent Assay (ELISA) Untuk mendeteksi Antibodi Mycoplasma GallisepticumI. Bogor : Balai penelitian veteriner, 2004.

Djamil R & Utami KN. Uji Penghambatan Enzim α-Glukosidase Secara In-Vitro Dari Ekstrak Etanol 70% Daun Leunca (Solanum nigrum L.). Rakernas & PIT IAI. 2016. pp 1-8.

Ahmad AR, Juwita J, Ratulangi SAD dan Malik A. Penetapan Kadar Fenolik Dan Flavonoid Total Ekstrak Metanol Buah Dan Daun Patikala (Etlingera Elatior (Jack) Rm Sm) Menggunakan Spektrofotometri Uv-Vis. Pharmaceutical Sciences And Research. 2016;2(1):1-10.

Sino Biological. ELISA encyclopdia (Internet). http://www.elisa-antibody.com/ELISA-introduction. 2017 (Cited 10 March 2017).

Suhaenah A. Uji Aktivitas Antioksidan Ekstrak Jamur Kancing (Agaricus Bisporus) dan Efek Inhibisinya Terhadap Enzim Alfa-Glukosidase (Tesis). Makassar: Program Pasca Sarjana Universitas Hasanuddin, 2016.




DOI: https://doi.org/10.56711/jifa.v12i1.619

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ISSN: 2085-4714 | e-ISSN: 2502-9444

Editor's Address:

Faculty of Pharmacy, Univeristas Muslim Indonesia
2nd Campus of UMI: Jl. Urip Sumoharjo km. 5 , Makassar, South Sulawesi, Indonesia

E-mail: jurnal.farmasi@umi.ac.id

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