AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAUN DAN AMPAS TEH HIJAU (Camellia sinensis L. ) TERHADAP BAKTERI PENYEBAB JERAWAT (Propionibacterium acne DAN Staphylococcus epidermidis ) SECARA DIFUSI AGAR
Abstract
Acne is one skin problem commonly known as acne vulgaris, that is a state where the skin pores are clogged. Some of the bakteria causing the growth on acne are Propianibacterium acne and Staphylococcus epidermidis . Green tea (Camellia sinensis L.) is one of the plants that can be useful as antibacterial. This study aimed to obtained the antibacterial activity of ethanol extract leaves and sregs of green tea which has the potential to inhibit the growth of acne causing bacteria by the agar diffusion method. The simplisia of green tea leaves and dregs are macerated with ethanol 96% and obtained the weight of ethanol extract of green tea leaves 34.6 grams. In determination of Minimum Inhibitory Concentration (MIC) ethanol extract of the leaves and the dregs, the MIC value obtained is the same as the two test bacteria on the concentration of 0.1%; whereas, KBM value is on the concentration of 0.4%. The result of the antibacterial activity of ethanol extract of green tea leaves at the largest concentration of 8% has an inhibitory zone diameter of 18.11 mm to Propianibacterium acne and 18.05 mm to Staphylococcus epidermidis. For the antibacterial activity test of ethanol extract the green tea dregs at the largest concentration of 8% has the largest average inhibitory zone diameter of 17.45 mm against Propinibacterium acne and 15.68 mm against Staphylococcus epidermidis.
Keywords
Full Text:
Fulltext PDF (Bahasa Indonesia)References
Saraswati FN. Uji Aktivitas Antibakteri Ekstrak Etanol 96% Limbah Kulit Pisang Kepok Kuning (Musa balbisiana) Terhadap Bakteri Penyebab Jerawat (Staphylococcus epidermidis, Staphylococcus aureus, dan Propionibacterium acne) (Skripsi).Jakarta: Fakultas Kedokteran dan Ilmu Kesehatan, Universitas Islam Negeri Syarif Hidayatullah, 2015.
Soetjipto H, Martono Y & Setiawan TH. Potensi Pemanfaatan Ekstrak Ampas Teh Hijau Fraksi Etil Asetat Sebagai Agensia Antibakteri. Seminar Nasional Kimia dan Pendidikan Kimia IV. 2012.pp. 388.
Widyaningrum N, Badie’ah, Lestari S. Antibacterial Activity of The Dregs of Green Tea Leaves (Camellia Sinensis L) on Staphylococcus epidermidis as Causes of Acne. Journal of Science Science Education. 2017;1(2):3.
Widyaningrum N. Epigallocatechin-3-Gallate (EGCG) Pada Daun Teh Hijau Sebagai Anti Jerawat. Majalah Farmasi dan Farmakologi. 2013;17(3):96.
Widyaningrum N, Fudholi A, Sudarsono & Setyowati EP. Stability of Epigallocatechin Gallate (EGCG) from Green Tea (Camellia sinensis) and its Antibacterial Activity against Staphylococcus epidermidis ATCC 35984 and Propionibacterium acnes ATCC 6919. Asian J. Biol. Sci. 20158(2).
Mile FA. Aktivitas Antibakteri Ekstrak Etanol Batang Phytocrene macrophylla Blume Dengan Metode Difusi Agar (Skripsi). Makassar: Fakultas Farmasi, Universitas Muslim Indonesia, 2016.
Efendi YN & Hertiani T. Potensi Antimikroba Ekstrak Etanol Sarang Semut (Myrmecodia tuberosa Jack.)Terhadap Candida albicans, Escherichia coli dan Staphylococcus aureus.Traditional Medicine Journal. 2013:18(1):56.
Davis & Stout. Disc Plate Method of Microbiological Antibiotic Essay. Journal of Microbiology. 1971;22(4).
DOI: https://doi.org/10.56711/jifa.v10i2.448
Refbacks
- There are currently no refbacks.
Indexed by:
ISSN: 2085-4714 | e-ISSN: 2502-9444
Editor's Address:
Faculty of Pharmacy, Univeristas Muslim Indonesia
2nd Campus of UMI: Jl. Urip Sumoharjo km. 5 , Makassar, South Sulawesi, Indonesia
E-mail: jurnal.farmasi@umi.ac.id