POTENSI EKSTRAK RUMPUT LAUT (Eucheuma cottoni) SEBAGAI ANTIOKSIDAN
Abstract
Seaweed (Eucheuma cottoni) is a marine biota that is often used as an additive to pharmaceutical products that function as thickener. But seaweed, which is rich in carrageenan, also contains vitamin C which has the potential as an antioxidant. The method used was to test the antioxidant activity of seaweed extract (Eucheuma cottoni) with the DPPH (1,1-Diphenyl-2-Picrylhdrazil) damping method. The test sample was in the form of seaweed ethyl acetate extract, where the standard standard of comparison of quarsetin and seaweed extract samples was made in 5 variations of concentration. The standard curve produced by quarsetin with concentrations of 2, 4, 6, 8, 10 ppm at the maximum wavelength DPPH 514,942 nm. The results obtained by the Kuarsetin standard with regression values y = 6.4765x + 7.567, the correlation value r = 0.99959 and IC50 = 6.48 (very strong antioxidant activity) while the ethyl acetate extract of seaweed with concentrations of 600, 800, 1000, 1200, 1400 ppm % inhibition was obtained with regression values y = 0.0104x + 16.708, the correlation value r = 0.9972 and IC50 = 3201.15 (very weak antioxidants). So it can be concluded that the seaweed ethyl acetate extract has the potential for antioxidant activity.
Keywords
Full Text:
FULLTEXT PDF (Bahasa Indonesia)References
Bawa IGAG, Bawa Putra AA dan Laila IR. Penentuan Ph Optimum Isolasi Karaginan Dari Rumput Laut Jenis Eucheuma Cottonii. Jurnal Kimia.2007;1(1): 15-20.
Thevanayagam H, Mohamed SM, Chu WL. Assessment of UVB-Photophrotective Activities of Carrageenan in Keratinocytes. Springer Science Business Media Dordrecht.2013: 1-7.
Ahmad AR, Mun’im A, Elya B. Study of antioxidant activity with reduction of free radical DPPH and xanthine oxidase inhibitor of the extract Ruellia tuberosa Linn Leaf. International Research Journal of Pharmacy.2012;3(11):66-70.
Huliselan YM, Runtuwene MRJ, Wewengkang DS. Aktivitas Antioksidan Ekstrak Etanol, Etil Asetat, Dan n-Heksan Dari Daun Sesewanua (Clerodendron squamatum Vahl.). Pharmacon.2015;4(3):155-163.
Day RA and Underwood AL. Analisis Kimia Kuantitatif. Diterjemahkan oleh Lis Sopyan. Jakarta: Penerbit Erlangga, 2002.
Salamah N, dan Erlinda W. Aktivitas Antioksidan Ekstrak Metanol Daun Kelengkeng (Euphoria longan (L) Steud.) Dengan Metode Penangkapan Radikal 2,2’-difenil-1-pikrilhidrazil. Pharmaҫiana. 2015;5(1):25-3.
Molyneux P. The Use Of The Stable Free Radical Diphenylpicrylhydrazyl (DPPH) For Estimating Antioxidant Activity. J. Sci.Techno.2004;26(2):211-219.
Husnah M, Barroroh H, Hayati EK. Identifikasi dan uji aktivitas golongan senyawa antioksidan ekstrak kasar buah pepino (Solanum muricatum Aiton) berdasarkan variasi pelarut (Skripsi) Malang : Universitas Islam Negeri Maulana Malik Ibrahim, 2009.
Rastuti U dan Purwanti. Uji Aktivitas Antioksidan Ekstrak Daun Kalba Dengan Metode DPPH dan Identifikasi Senyawa Metabolit Sekundernya. Molekul.2012;7(1):33-42.
Hanum F, Tarigan MA, Kaban IMD. Ekstraksi Pektin dari Kulit Buah Pisang Kepok (Musa paradisiaca). Jurnal Teknik Kimia USU. 2012;1(1): 49-53
Badarinath AV, RAo KM, Chetty CMS, Ramkanth V, Rajan TVS, Gnanaprakash K. A review on in-vitro antioxidant methods: comparisons, correlations and considerations. Int. J. PharmTech Res. 2010;2(2):1276–1285.
DOI: https://doi.org/10.56711/jifa.v12i1.600
Refbacks
- There are currently no refbacks.
Indexed by:
ISSN: 2085-4714 | e-ISSN: 2502-9444
Editor's Address:
Faculty of Pharmacy, Univeristas Muslim Indonesia
2nd Campus of UMI: Jl. Urip Sumoharjo km. 5 , Makassar, South Sulawesi, Indonesia
E-mail: jurnal.farmasi@umi.ac.id